Genetic variation and drought response in two Populus x euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants.
Identifieur interne : 003615 ( Main/Exploration ); précédent : 003614; suivant : 003616Genetic variation and drought response in two Populus x euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants.
Auteurs : Ludovic Bonhomme [France] ; Romain Monclus ; Delphine Vincent ; Sabine Carpin ; Stéphane Claverol ; Anne-Marie Lomenech ; Valérie Labas ; Christophe Plomion ; Franck Brignolas ; Domenico MorabitoSource :
- Phytochemistry [ 1873-3700 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Populus.
- génétique : Feuilles de plante, Populus.
- métabolisme : Eau, Populus.
- physiologie : Feuilles de plante.
- Protéomique, Stress oxydatif, Sécheresses, Variation génétique, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Water.
- enzymology : Populus.
- genetics : Plant Leaves, Populus.
- metabolism : Populus.
- physiology : Plant Leaves.
- Droughts, Electrophoresis, Gel, Two-Dimensional, Genetic Variation, Oxidative Stress, Proteomics.
Abstract
Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit, about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.
DOI: 10.1016/j.phytochem.2009.05.013
PubMed: 19560791
Affiliations:
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Lomenech</name></author><author><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valérie" last="Labas">Valérie Labas</name></author><author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name></author><author><name sortKey="Brignolas, Franck" sort="Brignolas, Franck" uniqKey="Brignolas F" first="Franck" last="Brignolas">Franck Brignolas</name></author><author><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="eISSN">1873-3700</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Droughts (MeSH)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (physiology)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Proteomics (MeSH)</term><term>Water (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Eau (métabolisme)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (physiologie)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéomique (MeSH)</term><term>Stress oxydatif (MeSH)</term><term>Sécheresses (MeSH)</term><term>Variation génétique (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Water</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Eau</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Droughts</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Genetic Variation</term><term>Oxidative Stress</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Protéomique</term><term>Stress oxydatif</term><term>Sécheresses</term><term>Variation génétique</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit, about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19560791</PMID><DateCompleted><Year>2009</Year><Month>08</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3700</ISSN><JournalIssue CitedMedium="Internet"><Volume>70</Volume><Issue>8</Issue><PubDate><Year>2009</Year><Month>May</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Genetic variation and drought response in two Populus x euramericana genotypes through 2-DE proteomic analysis of leaves from field and glasshouse cultivated plants.</ArticleTitle><Pagination><MedlinePgn>988-1002</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2009.05.013</ELocationID><Abstract><AbstractText>Genotype and water deficit effects on leaf 2-DE protein profiles of two Populus deltoides x Populus nigra, cv. 'Agathe_F' and 'Cima', were analysed over a short-term period of 18 days in glasshouse using 4-month-old rooted cuttings and over a long-lasting period of 86 days in open field using 4-year-old rooted cuttings. Leaf proteomes were analyzed using two-dimensional gel electrophoresis, and proteins were identified after database searching from MS peptide spectra. A reliable genotype effect was observed in the leaf proteome over experiment locations, water regimes and sampling dates. Quantitative differences between genotypes were found. Most of them corresponded to proteins matching isoforms or post-translational modification variants. However, 'Cima' displayed the highest abundance of antioxidant enzymes. In response to water deficit, about 10% of the reproducible spots significantly varied regardless of the experiment location, among which about 25% also displayed genotype-dependent variations. As a whole, while 'Cima' differed from 'Agathe_F' by increased abundance of enzymes involved in photorespiration and in oxidative stress, 'Agathe_F' was mainly differentiated by increased abundance of enzymes involved in photosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bonhomme</LastName><ForeName>Ludovic</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Université d'Orléans, UFR-Faculté des Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, rue de Chartres, BP 6759, F-45067 Orléans Cedex 02, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monclus</LastName><ForeName>Romain</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Vincent</LastName><ForeName>Delphine</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Carpin</LastName><ForeName>Sabine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Claverol</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lomenech</LastName><ForeName>Anne-Marie</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Labas</LastName><ForeName>Valérie</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Plomion</LastName><ForeName>Christophe</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Brignolas</LastName><ForeName>Franck</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Morabito</LastName><ForeName>Domenico</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>06</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055864" MajorTopicYN="N">Droughts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>05</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>05</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>8</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19560791</ArticleId><ArticleId IdType="pii">S0031-9422(09)00208-8</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2009.05.013</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li></region><settlement><li>Orléans</li></settlement><orgName><li>Université d'Orléans</li></orgName></list><tree><noCountry><name sortKey="Brignolas, Franck" sort="Brignolas, Franck" uniqKey="Brignolas F" first="Franck" last="Brignolas">Franck Brignolas</name><name sortKey="Carpin, Sabine" sort="Carpin, Sabine" uniqKey="Carpin S" first="Sabine" last="Carpin">Sabine Carpin</name><name sortKey="Claverol, Stephane" sort="Claverol, Stephane" uniqKey="Claverol S" first="Stéphane" last="Claverol">Stéphane Claverol</name><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valérie" last="Labas">Valérie Labas</name><name sortKey="Lomenech, Anne Marie" sort="Lomenech, Anne Marie" uniqKey="Lomenech A" first="Anne-Marie" last="Lomenech">Anne-Marie Lomenech</name><name sortKey="Monclus, Romain" sort="Monclus, Romain" uniqKey="Monclus R" first="Romain" last="Monclus">Romain Monclus</name><name sortKey="Morabito, Domenico" sort="Morabito, Domenico" uniqKey="Morabito D" first="Domenico" last="Morabito">Domenico Morabito</name><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name><name sortKey="Vincent, Delphine" sort="Vincent, Delphine" uniqKey="Vincent D" first="Delphine" last="Vincent">Delphine Vincent</name></noCountry><country name="France"><region name="Centre-Val de Loire"><name sortKey="Bonhomme, Ludovic" sort="Bonhomme, Ludovic" uniqKey="Bonhomme L" first="Ludovic" last="Bonhomme">Ludovic Bonhomme</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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